diff --git a/Lib/importlib/resources/_common.py b/Lib/importlib/resources/_common.py
index 147ea19188f..ca1fa8ab2fe 100644
--- a/Lib/importlib/resources/_common.py
+++ b/Lib/importlib/resources/_common.py
@@ -67,7 +67,10 @@ def from_package(package):
 
 
 @contextlib.contextmanager
-def _tempfile(reader, suffix=''):
+def _tempfile(reader, suffix='',
+              # gh-93353: Keep a reference to call os.remove() in late Python
+              # finalization.
+              *, _os_remove=os.remove):
     # Not using tempfile.NamedTemporaryFile as it leads to deeper 'try'
     # blocks due to the need to close the temporary file to work on Windows
     # properly.
@@ -81,7 +84,7 @@ def _tempfile(reader, suffix=''):
         yield pathlib.Path(raw_path)
     finally:
         try:
-            os.remove(raw_path)
+            _os_remove(raw_path)
         except FileNotFoundError:
             pass
 
diff --git a/Misc/NEWS.d/next/Library/2022-05-31-14-58-40.gh-issue-93353.9Hvm6o.rst b/Misc/NEWS.d/next/Library/2022-05-31-14-58-40.gh-issue-93353.9Hvm6o.rst
new file mode 100644
index 00000000000..67be3c68f47
--- /dev/null
+++ b/Misc/NEWS.d/next/Library/2022-05-31-14-58-40.gh-issue-93353.9Hvm6o.rst
@@ -0,0 +1,3 @@
+Fix the :func:`importlib.resources.as_file` context manager to remove the
+temporary file if destroyed late during Python finalization: keep a local
+reference to the :func:`os.remove` function. Patch by Victor Stinner.